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Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in Phylum Chloroflexi
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  • Sarah Schwartz,
  • Lily Momper,
  • L. Thiberio Rangel,
  • Cara Magnabosco,
  • Jan Amend,
  • Gregory Fournier
Sarah Schwartz
Massachusetts Institute of Technology
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Lily Momper
Massachusetts Institute of Technology
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L. Thiberio Rangel
Massachusetts Institute of Technology
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Cara Magnabosco
ETH Zurich
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Jan Amend
University of Southern California
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Gregory Fournier
Massachusetts Institute of Technology
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Abstract

Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain architecture in members of Phylum Chloroflexi. Phylogenetic analyses of protein domains within Nir indicate that an ancestral horizontal transfer and fusion event produced this chimeric domain architecture. We also identify an expanded genomic diversity of a rarely reported nitric oxide reductase subtype, eNOR. Together, these results suggest a greater diversity of denitrification enzyme arrangements exist than have been previously reported.
30 Apr 2021Submitted to MicrobiologyOpen
30 Apr 2021Submission Checks Completed
30 Apr 2021Assigned to Editor
07 May 2021Reviewer(s) Assigned
07 Jun 2021Review(s) Completed, Editorial Evaluation Pending
11 Jun 2021Editorial Decision: Revise Minor
25 Aug 20211st Revision Received
25 Aug 2021Submission Checks Completed
25 Aug 2021Assigned to Editor
25 Aug 2021Review(s) Completed, Editorial Evaluation Pending
25 Aug 2021Reviewer(s) Assigned
27 Sep 2021Editorial Decision: Revise Minor
07 Nov 20212nd Revision Received
08 Nov 2021Submission Checks Completed
08 Nov 2021Assigned to Editor
09 Nov 2021Review(s) Completed, Editorial Evaluation Pending
10 Nov 2021Reviewer(s) Assigned
26 Nov 2021Editorial Decision: Revise Minor
02 Dec 20213rd Revision Received
02 Dec 2021Submission Checks Completed
02 Dec 2021Assigned to Editor
02 Dec 2021Review(s) Completed, Editorial Evaluation Pending
02 Dec 2021Editorial Decision: Accept
Feb 2022Published in MicrobiologyOpen volume 11 issue 1. 10.1002/mbo3.1258