A novel high gain flexible receiving antenna is proposed, which can be used for microwave wireless power transmission(MWPT). The antenna consists of a three-layer toroidal structure in a generalized iterative design with a coplanar waveguide(CPW) feed, and the radiator is integrated on Kapton substrate. In order to improve the gain of the antenna, an air-gap and flexible reflector are loaded at the back of the antenna. The antenna has a peak gain of 9.51dBi at 5.8 GHz. To characterize the stability of the antenna, the fabricated prototype uses three configurations of 85 mm, 60 mm and 45 mm respectively. The S11 of the antenna remains < -10dB, and the maximum harvesting efficiency is 71.3%.

Aim: Although current guidelines recommend 3-6 months of dual antiplatelet therapy (DAPT) for patients without indications for anticoagulation after transcatheter aortic valve replacement (TAVR), evidence-based evidence was lacking. Our aim was to review the most recent evidence comparing the safety and efficacy of both treatment modalities, single antiplatelet therapy (SAPT) and DAPT, after TAVR. Methods: We systematically searched the literature on Embase, PubMed, Cochrane and Medline until January 5, 2022. Our primary outcome indicators were: all-cause mortality, stroke and total bleeding rates, and secondary outcome indicators were: cardiovascular mortality, myocardial infarction (MI) and the incidence of major and life-threatening bleeding (LTB). A random-effects model was used, and subgroup analyses were performed according to study type and follow-up time. Results: A total of 14 studies (4 RCTs, 10 observational studies) involving 21,546 patients were finally screened. Compared with the DAPT treatment modality, patients in the SAPT group showed a significant difference in short-term cardiovascular mortality after TAVR (RR [95% CI] =0.39 [0.19-0.78], P = 0.008) and a significantly lower risk of bleeding (RR [95% CI] =0.56 [0.48-0.65], P <0.001). There was also a significant difference in the incidence of major bleeding and LTB (RR [95% CI] =0.59[0.47-0.76], P <0.0001), but no statistically significant difference in other aspects. Conclusion: Compared with DAPT treatment modalities, the choice of SAPT for patients without anticoagulation indication after TAVR better reduces the risk of postoperative bleeding and short-term cardiovascular death without increasing the incidence of all-cause mortality, stroke, and MI.

Dietary metabarcoding has vastly improved our ability to analyse the diets of animals, but it is hampered by a plethora of technical limitations including potentially reduced data output due to the disproportionate amplification of the DNA of the focal predator, here termed ‘the predator problem’. We review the various methods commonly used to overcome this problem, from deeper sequencing to exclusion of predator DNA during PCR, and how they may interfere with increasingly common multi-predator-taxon studies. We suggest that multi-primer approaches with an emphasis on achieving both depth and breadth of prey detections may overcome the issue to some extent, although multi-taxon studies require further consideration, as highlighted by an empirical example. We also review several alternative methods for reducing the prevalence of predator DNA that are conceptually promising but require additional empirical examination. The predator problem is a key constraint on molecular dietary analyses but, through this synthesis, we hope to guide researchers in overcoming this in an effective and pragmatic way.

Giant clams are keystone species on coral reefs, but global demand for their harvest has decimated populations and resulted in all Tridacnids being listed on both CITES and IUCN lists. However, giant clams are notoriously difficult to identify, and recent molecular work has revealed that morphological misidentification of giant clams have confounded current population assessments and extinction risk. The most recent study of the status of giant clams in the Samoan Archipelago was published over 20 years ago, without molecular corroboration of visual identifications. Using morphologic characteristics and ez-RAD genetic techniques, we identify the existence of Tridacna noae in the Samoan archipelago, presenting the first observation and a resulting range expansion. Accurately identifying the extant species in the archipelago is the first step towards a much-needed population status assessment to effectively manage these long-lived species.

Attempts over the past 30 years to explain geographic variation in the strength of herbivore pressure gave rise to the latitudinal herbivory hypothesis. However, this long-standing hypothesis has rarely been tested using community-level data. In this study, we selected 43 grassland sites along a 1,500-km latitudinal gradient (c. 27°N to 39°N) on the Qinghai-Tibetan Plateau. We calculated community-wide herbivory at each site by summing herbivory across plant species weighted by the biomass of each species; we then investigated how abiotic and biotic latitudinal correlates drove community-wide herbivory via their effects on intraspecific herbivory variability and species’ turnover. We found that community-wide herbivory decreased with latitude, mirroring intraspecific herbivory variability. Furthermore, intraspecific herbivory variability was driven by climatic factors, but not edaphic or plant community factors. Overall, our study highlights the importance of considering both intraspecific herbivory variability and species’ turnover for predicting how climate change will alter community-wide herbivory.

The study by Worck et al. raises interesting findings with regard to left atrial posterior wall ablation. The utility of ablation at the CRZ -- which may represent epicardial connection via the septopulmonary bundle -- warrants future research. Upcoming trials utilising existing technology, along with increased availability of pulsed field ablation, will advance our knowledge of the impact of left atrial posterior wall isolation.

Co-immobilization of multiple proteins onto one nanosupport has large potential in mimicking natural multiprotein complexes and constructing efficient cascade biocatalytic systems. However, control of different proteins regarding their spatial arrangement and loading ratio remains a big challenge, and protein co-immobilization often requires the use of purified proteins. Herein, built upon our recently designed SpyTag-functionalized magnetic nanoparticles (MNP), we established a modular MNP platform for site-specific, tunable, and cost-effective protein co-immobilization. SpyCatcher-fused enhanced green fluorescent protein (i.e., EGFP-SpyCatcher) and mCherry red fluorescent protein (i.e., RFP-SpyCatcher) were designed and conjugated on MNP, and the immobilized proteins showed 3~7-fold enhancement in storage stability and greatly improved stability against freeze-thaw process compared to free proteins. The protein-conjugated MNP also retained desirable colloidal stability and magnetic responsiveness, enabling facile proteins recovery. Also, one-pot co-immobilization of the two proteins could be fine-tuned with their feed ratios. In addition, MNP could selectively and efficiently co-immobilize both SpyCatcher-fused proteins from combined cell lysates without purification, offering a convenient and cost-effective approach for multiprotein immobilization. This MNP platform provides a facile and efficient tool to construct bio-nano hybrid materials (i.e., protein-based MNP) and multienzyme systems for industrial and biotechnological applications.

A 50-year-old female presented with a history of fatigue after activities. Transthoracic echocardiography showed a 2.8 cm dilatation at the ostium of the left coronary artery and a 7.1×7.4 cm cyst-like aneurysm at the upper back of the left atrium, interlinking an anomalous fistula from the left coronary artery to the right atrial appendage, accompanied by moderate mitral and tricuspid regurgitation. Cardiac 3D-computed tomography identified the diagnosis of ruptured aortic sinus aneurysm (RASA) and a giant left coronary artery aneurysm fistula to the right atrium. Transesophageal echocardiogram and surgical vision confirmed these findings. Surgeries including RASA repair, coronary artery bypass grafting, and mitral and tricuspid valvuloplasty were then performed. Procedures proceeded successfully, the patient was fully recovered and discharged home.

A 49-year-old male presented to hospital with symptoms of acute cerebral infarction and pulmonary embolism who underwent transcatheter closure of atrial septal defect a year ago. Transthoracic echocardiography showed a 13×9 mm hypoechoic mass attached to the left-atrial side of the device, which was suspected to be neoplasm or thrombus. The patient was indicated for surgery after multidisciplinary discussion due to ineffective medical therapy and typical stroke and pulmonary embolism symptoms. Three-dimensional transesophageal echocardiography (3D-TEE) revealed left-atrial vegetation (21×16 mm) and right-atrial vegetation (8×6 mm) attached to the device, which were confirmed as thrombus by surgical separation and laboratory examination. This case highlights the importance of 3D-TEE and a multidisciplinary team in the diagnosis and therapy of device-related thrombus.

We describe a 13-year-old boy with a bilateral hemorrhagic lesion in basal ganglia related to mild traumatic brain injury. Due to the severity of the traumatic brain injury, we did not expect the injury to either side of the basal ganglia. So tha

Peripartum cardiomyopathy is an idiopathic cardiomyopathy that most commonly affects women in the third trimester of pregnancy or in the first five months of the postpartum period. Peripartum cardiomyopathy is associated with a myriad of different findings on electrocardiograms. There is also a risk of supraventricular tachycardia, atrial fibrillation, atrial flutter, and ventricular arrhythmias. This review discusses the prevalence and management of tachyarrhythmias and bradyarrhythmias in peripartum cardiomyopathy.

A detailed analysis of the blazars detected by the Fermi/LAT (3LAC) and (4LAC) along with the 𝛾 −ray Narrow line – Seyfert 1 Galaxies (𝛾-NLSy1s) has been provided here with emphasize to the weak anti-correlation between synchrotron peak frequency and peak luminiosity, jet kinetic power and synchrotron peak frequency along with the properties of FSRQs, BL Lacs (Chen et al, 2020) with the central engine properties that is, the accretion luminosity (𝐿𝑑𝑖𝑠𝑘 ) and black hole mass (𝑀𝐵𝐻 ) where study has been done regarding the broad emission spectral line systems along with the association of more massive black holes with absorption line systems where computations has been made in Eddington units (𝐿𝑑𝑖𝑠𝑐 /𝐿𝐸𝑑𝑑 ) having the broad line objects to have a higher accretion rate (𝐿𝑑𝑖𝑠𝑐 /𝐿𝐸𝑑𝑑 > 0.01) with the sources being identified in recurrence with the HighCompton Dominated (HCD-CD > 1) and Low-Compton Dominated (LCD- CD ≲ 1) objects where according to (Paliya et al, 2021) the physical properties of the Fermi Blazars are to be controlled by the accretion rate in Eddington units. From the known redshifts of the Fermi satellite data, the spectral energy distributions (SEDs) have been computed where the proton dominated jet powers and the luminosity has been computed which is of the same order of (or slightly larger than) the disc luminosity (Ghisellini et al, 2010). In case of Flat Spectrum Radio quasars (FSRQs), the high energy peak of the SED increases with the luminosity where the X-ray spectra becomes harder and harder for larger luminosities, however in case of BL Lacs, they become redder with a softer 𝛾-ray slope and high energy for peak luminosities. For all the blazars (FRSQs + BL Lacs), the higher luminosity bin is vastly populated by FRSQs while the lower luminosity bin is populated by BL Lacs (Ghisellini et al, 2017). In the Fermi LAT – Bright AGN Sample (LBAS) by combining the Fermi 𝛾-ray spectra with Swift, optical, infra-red, radio, hard X-ray, 𝛾-ray data it has been observed with accuracy that in case of 50% of the radio bright high energy peaked BL Lacs (HBL) detected in the LABS Sample, only less than 13% of the known FSRQs and LBL BL Lacs are included. The synchrotron peak frequency (𝑣𝑝𝑒𝑎𝑘 𝑠 ) has been positioned between 1012.5 and 1014.5 Hz in FSRQs and between 1013 and 1017 Hz in featureless BL Lac objects. The LAC detector being more sensitive to flat spectrum 𝛾-ray sources, the exact correlation between 𝑣𝑝𝑒𝑎𝑘 𝑠 and 𝛾-ray spectral index highly favors the detection of the high energy peaked blazars having the Fermi overabundance compared to radio and Energetic Gamma Ray Experiment Telescope (EGRET) samples. This in turn makes a selection effect where in the soft X-ray band, HBL BL Lacs are the most dominant types of blazars (Abdo et al, 2010).

Pavement distress classification is a vital step for automatic pavement inspection and maintenance. Recently, patch-based approaches have achieved promising performances and thus extensive attention in this field. However, these methods simply assume that all patches contribute equally to the distress classification, leading to weakly discriminating abilities of models. Moreover, their tedious processes also leads to a low efficiency in inference. In this letter, we present a novel patch-based pavement distress classification approach named Deep Patch Soft Selective Learning (DPS$^2$L), which addresses these issues. Similar to other patch-based approaches, DPS$^2$L partitions the pavement images into patches and aggregates the patch features to accomplish the task. To address the first issue, we introduce a succinct Soft Patch Feature Selection Network (SPFSN) to assess the importance of each patch to the distress classification with a score based on its feature. These scores will be considered as patch-wise weights for feature aggregation. In such a manner, the most discriminative patches are selected in a soft way, and thereby benefit the final classification. To address the inference efficiency issue, knowledge distillation is leveraged to transfer the classification knowledge from DPS$^2$L to the image-based approaches, such as EfficientNet-B3. This distilled model enables incorporating both the advantages of patch-based approaches in classification performance and the advantages of image-based approaches in inference efficiency. Extensive experiments on a large-scale pavement image dataset named CQU-BPDD demonstrates the superiority of our methods over baselines regardless of performance or efficiency.

Understanding sedimentation and runoff variations caused by land use change have emerged as important research areas, due to the ecological functions of landscape patterns. The aims of this study were to determine the relationship between landscape metrics (LMs), runoff, and sedimentation and explore the crucial LMs in the watersheds on the Loess Plateau. From 1985 to 2010, Grassland (GRA) was the dominant landscape in the Tuweihe (TU) and Gushanchuan (GU) watersheds. Unused land (UNL) and farmland (FAR), respectively, experienced the greatest transformations. The landscape in the study area tended to become regular, connected, and aggregated. The landscape stability of the TU watershed was higher than that of the GU watershed. Annual runoff and sedimentation gradually decreased and significant relationship was found between them (P <0.01). Due to larger FAR area and lower landscape stability in the GU watershed, the sedimentation of the two watersheds were similar, even though the runoff in the TU watershed was greater. The LMs had more significant effects on runoff than that on sedimentation yield. Shannon’s evenness index and the patch cohesion index had the greatest effects on runoff and sedimentation, making them the key factors of influencing water and soil loss. which could provide a scientific basis for the prevention and treatment of soil erosion.

A mixed breed dog was treated for severe hemolytic anemia secondary to Babesia canis infection. Within 14 hours of hospitalization, the dog developed respiratory distress and hypoxemia. The patient improved with lung-protective mechanical ventilation and neuromuscular blockade. The dog survived to discharge and was reportedly healthy at three-month follow up.

Cutis laxa is a connective tissue disease, which is either inherited or acquired with various clinical presentation. Increased level of MMP-2 and MMP-9 might be associated with the cutis laxa disease. However, our findings in current experience need to be validated in larger clinical settings.

Phytoplasma can infect thousands of plants and caused huge economic losses around the world. The large-scale spread and serious lethality of phytoplasma prompt the urgent need for sensitive, accurate, visual and rapid detection of these pathogens. Current molecular assays used for detecting phytoplasma are expensive and time consuming. Here, we established a novel All-In-One Dual (AIOD) CRISPR detection platform based on CRISPR/LbCas12a technology and Recombinase Polymerase Amplification (RPA) for the diagnosis of multiple phytoplasma. The protocol is simple, requiring one vessel, rapid and sensitive, and the output is visual. Cas12a/crRNAs complexes are added into a reaction containing RPA Mix, RPA Primers and single-stranded DNA fluorophore-quencher (ssDNA-FQ). All components, including 1 μL of sample DNA, are added together and then incubated in one tube at 37 °C. Phytoplasma was detected after 15 min or less from leaf harvest. Positive results can be observed by the naked eye via fluorescent signals. We optimized the amounts of crRNA, LbCas12a and the ssDNA fluorophore in the detection system. Finally, an optimized system was established containing 1,000 nM ssDNA-FQ and a 2:1:1 ratio of LbCas12a/crRNA1/crRNA2 complex with a 0.8 μM concentration as 1. In the optimized reaction, the AIOD-CRISPR detection system exhibited high sensitivity, with limits of detection reaching 3.37E + 2 copies of phytoplasma DNA per reaction. Field tests indicated the AIOD-CRISPR detection system possessed high specificity and reached the 100% accuracy when compared with PCR detection. In conclusion, the AIOD-CRISPR detection system is a ideal selection with high specificity and sensitivity for phytoplasma detection. Our work provides a technique that can be potentially used to rapidly and simultaneously detect more pathogens.

Granulocytic sarcoma (GS) is a rare extramedullary tumor of immature myeloid cells and is frequently associated with acute or chronic leukemias [1]. In our case the tumor localization was pariatotemporal as it is not usuall presentation. ; we presented this case report to refresh the data of granuloctic sarcoma

The appearance for viruses that evolve to adapt to a new living niche often reflect on viral sequence changes. Fixation of these changes may require a long time through repeated transmission, thereby rendering a reduced size of an effective population harboring dominant alterations in their sequence spaces. Those approaches, with which we can closely monitor and survey the transient changes of viral sequences over the longer timescales, thus become a requisite to better understand the evolution of viral pathogenicity. Molecular barcodes are a powerful and practical molecular tool to individually label sequences, allowing for correcting sequencing errors and identifying true mutants of interest with a single nucleotide resolution. Molecular barcoding has also been implemented as a useful approach to study several zoonotic viruses. In this review, the emphasis will not only be limited to summarize current studies focusing on viral pathogenesis and fitness; we will also propose ideas that molecular barcodes can be used to execute surveillance of changes of viral sequences. We believe that this review will be helpful for the readers to better understand the rationale and the usage of molecular barcodes and the perspectives of what molecular barcodes can do for fighting upcoming emerging infectious diseases.

The structural study of lignin-carbohydrate complexes (LCCs) provides a theoretical basis for the high-value utilization of biomass which has always been a hot topic in biomaterials and biomass refining. This paper compares the structure of two types of LCCs in poplar. After poplar wood was crushed by ball milling, water-soluble LCCs and the Björkman LCCs were extracted by hot water extraction and the Björkman classical method, respectively. The molecular weight was determined by GPC, the composition was determined by chemical analysis, and the structure was studied by UV, IR and 2D-NMR. Comparing the two, the results showed that water-soluble LCCs contains a higher content of carbohydrates and has a relatively wide molecular weight distribution, while the carbohydrate content of the Björkman LCCs was relatively low, and the molecular weight distribution range was relatively narrow. UV, IR and 2D-NMR results confirmed that the main chemical links between the lignin units of the two LCCs were β-O-4. β-β, β-5 structures. The chemical linkages between lignin and carbohydrates in water-soluble LCCs were mainly phenyl glycosidic bonds and ester bonds, while in the Björkman LCCs, there were not only have phenyl glycosides bonds and ester bonds, but also found existence of ether bonds between lignin and carbohydrates. This work contributes to a comprehensive understanding of the structure of poplar LCCs and provides theoretical support for the further application of poplar wood.

β-Nicotinamide mononucleotide (NMN) is an important precursor in the synthesis of nicotinamide adenine dinucleotide (NAD+) and confers multiple health benefits, resulting in the rapid growth of NMN market capacity in the fields of food and health care. To overcome the drawbacks of NMN production by the existing chemical or microbial fermentation method, there is an urgent need to develop a prospective NMN production strategy with low cost, low pollution, and high yield. In this study, we demonstrated an artificial in vitro multi-enzyme cascade biocatalysis using starch and nicotinamide (Nam) as substrates for the synthesis of NMN in one-pot. This multi-enzyme cascade reaction was optimized in terms of pH value, buffer concentration, inorganic phosphate concentration, enzyme composition, and phosphoenolpyruvate concentration. Under optimized conditions, a high molar yield of 87.8% for NMN was achieved using 3.2 mM Nam as substrate, and a molar yield of 55.37% for NMN was also achieved under the initial Nam concentration of 9.21 mM. This in vitro enzymatic platform provides a promising and environmental friendliness biomanufacturing technology for the production of NMN.

Case ReportAn unusual case of huge cervical fibroid with tubercular aortic nodes in case of aortic occlusion.Large adnexal mass with leriche syndromeDr. Ujwala Umesh Netam , Dr. Umesh NetamDr. Uday PawarSun Hospital, Malad (W), Mumbai, IndiaAbstract: We report a 29-year-old female who had coexistence of huge cervical fibroid with aortic nodes of tubercular origin with aortic occlusion due to chronic thrombus of lower abdominal aorta.Keywords: Fibroid Uterus, Malignant / Tubercular aortic node, Aortic occlusion, Chronic thrombus of lower abdominal aorta with collaterals.

Background: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) modulators have varying levels of benefit regarding weight gain and growth, ultimately improving lung function and decreasing mortality rates in Cystic Fibrosis (CF) patients. The newly approved triple combination therapy (TCT) has shown weight gain benefits in clinical trials, but its long-term effects have yet to be studied in a site-wide setting. Methods: This retrospective study of 106 adult and pediatric CF patients on triple combination therapy for a year. We measured Body Mass Index (BMI) and BMI percentile changes and compared changes before and one year after initiation of TCT Results: TCT use showed weight gain over one year by increasing BMI in adult patients by 1.48 kg/m 2 (p-value < 0.0001). Pediatric patients saw significant benefit in BMI percentile with an average gain of 8.34 percentile (p-value= 0.0047). Discussion: The results of this study suggest that the new triple combination therapy improves BMI and BMI percentile in CF patients. This finding will help future CFF guidelines navigate the era of new modulators and the changes in baseline health that come with it.

{In this study, we present a second-order time-accurate unconditionally stable numerical method for a gradient flow for the Modica–Mortola functional with an equispaced multiple well potential. The proposed second-order time-accurate unconditionally stable numerical method is based on the operator splitting method (OSM). The nonlinear and linear terms in the gradient flow are solved analytically and using the Fourier spectral method, respectively. The numerical solutions in each step are bounded for any time step size and the overall scheme is temporally second-order accurate. We prove theoretically the unconditional stability and boundedness of the numerical solutions. In addition, several numerical tests are conducted to demonstrate the performance of the proposed method.