Objective: To explore the scope of antimicrobial stewardship activities based on the point prevalence study of antimicrobials among hospitalized patients in a tertiary-level care hospital in the UAE. Methods: A single-center cross-sectional prevalence study was conducted among inpatients admitted to a tertiary care hospital using the European Surveillance of Antimicrobial Consumption tool in February 2023. Antimicrobials used against bacteria only were surveyed. All patients admitted to the selected departments at 8 AM on the day of the survey were included. Data were described in numbers and percentages. Results: Among 136 patients admitted at the time of the study, 87 received antimicrobials, representing a prevalence of 64%. A total of 144 antimicrobial prescriptions were prescribed in 87 patients. The majority of the antimicrobials were administered parenterally (n=122, 84.7%), and over 60% (n=53) of the patients on antimicrobials received two antimicrobials. The majority of antimicrobials used for therapy were empiric in nature (n=68, 89.5%). Over half of the patients receiving antimicrobials for surgical prophylaxis were prescribed antimicrobials for two or more days. More than 60% of the antimicrobial orders were adherent to the hospital guidelines. Conclusion: The point prevalence of the use of antimicrobials is high, which highlights the need for more efficient and targeted stewardship efforts in improving adherence with the hospital guidelines. Wide usage of broadspectrum antimicrobials is observed.

Background: Intracardiac echocardiography (ICE) is increasingly used during left atrial appendage occlusion (LAAO) as an alternative to transesophageal echocardiography (TEE) Aim: To evaluate the impact of ICE vs. TEE guidance during LAAO on procedural characteristics and acute outcomes, as well the presence of peri-device leaks and residual septal defects during follow-up. Methods: All studies comparing ICE-guided vs. TEE-guided LAAO were identified. The primary outcomes were procedural efficacy and occurrence of procedure-related complications. Secondary outcomes included lab efficiency (defined as a reduction in in-room time), procedural time, fluoroscopy time, and presence of peri-device leaks and residual interatrial septal defects (IASD) during follow-up. Results: Twelve studies (n=5637) were included. There were no differences in procedural success group (98.3% vs. 97.8%; OR 0.73, 95% CI 0.42-1.27, p=0.27; I2=0%) or adverse events (4.5% vs. 4.4%; OR 0.81 95% CI 0.56-1.16, p=0.25; I2=0%) between the ICE-guided and TEE-guided groups. ICE guidance reduced in in-room time (mean-weighted 28.6-minute reduction in in-room time) without differences in procedural time or fluoroscopy time. There were no differences in peri-device leak (OR 0.93, 95% CI 0.68-1.27, p=0.64); however, an increased prevalence of residual IASD was observed with ICE-guided vs. TEE-guided LAAO (46.3% vs. 34.2%; OR 2.23, 95% CI 1.05-4.75, p=0.04). Conclusion: ICE guidance is associated with similar procedural efficacy and safety, but could result in improved lab efficiency (as established by a significant reduction in in-room time). No differences in the rate of periprocedural leaks were found. A higher prevalence of residual interatrial septal defects was observed with ICE guidance.

This study focuses on the evolution of the fatigue strength of L-PBF produced Ti-6Al-4V as a function of the chemical etching finishing process. The aim is to identify the critical fatigue crack initiation mechanisms and the transitions between them in terms of the evolution of the surface micro-geometry caused by the finishing process. This has been done using three different geometries and six different surface states, including the machined reference surface state. The evolution of the crack initiation mechanisms, identified via SEM observations of the fatigue failure surfaces, is then used to explain the evolutions of the fatigue strength and the fatigue scatter, in both the finite and the high cycle fatigue-life domains. As expected, chemical etching effects the fatigue life via a polishing effect on the micro-geometry, which directly influences both the finite and the high cycle fatigue domains. It is shown that chemical etching makes it possible to obtain fatigue strengths that are almost similar to those of the machined surface, despite the fact that the roughness is higher, even after an optimal etching time. However, it is also observed that etching cannot fully counter the effects of large surface connected porosities (typically lack-of-fusion) that lead to large surface cavities. Some minor scale or size effects can also be noted between the different coupon geometries. This does not modify the conclusions concerning the initiation mechanisms and their effect on the fatigue life.

Aims: Although alcohol consumption is proposed to trigger electrographic abnormalities, acute changes in arrythmias and conductance disturbances have not been systematically studied in young adults who are the age group most likely to binge drink. The aim of this study was to determine if changes in breath alcohol content (BrAC) during an acute binging episode influence electrographic abnormalities in young adults. Methods: Fifty-five university students (20.6±2.1 years of age), who were not regular users of alcohol, consumed 60% mixer and 40% vodka until 0.08% BrAC was reached (6.4±1.9 standard drinks). Five-lead Holter recordings were analysed by a blinded cardiac technician. Electrographic abnormalities were identified and classified according to location (supraventricular or ventricular) and type (arrhythmia or conduction disturbance). Results: Although heart rate was elevated with BrAC beyond 0.05% compared to 0.00%, no differences existed in the relative incidence of electrographic abnormalities by location or type. Logistic regression revealed that baseline electrographic abnormality significantly contributed to predicting electrographic abnormality in 0.00% to 0.05% (p=0.02) and 0.05% to 0.08% BrAC phases (p=0.01). Conclusions: Alcohol consumption, resulting in BrAC of 0.08%, increased heart rate after BrAC reached 0.05% but did not acutely influence the relative incidence of electrographic abnormality by location or type in apparently healthy young people. These results do not support the hypothesis that a binge drinking episode causes electrographic abnormalities in young adults.

Isophthalic acid (IPA) is a star phosphorescence molecule, which is a commercially available raw material with simple chemical structure, but is of good phosphorescence performance. However, the great research vacancy is still maintained because of its limited applications. Absolutely, polymer-matrix strategy is a common method to produce phosphorescence amorphous material by dispersing IPA into matrix such as polyvinyl alcohol (PVA). But how to further optimize the phosphorescence property of the resulted doping material (PVA-IPA)? Up to now, this question is new and very little attention has been paid. In this work, a co-doping strategy was explored, that is, charging the second dopant of ClCH2COOH into PVA-IPA was verified as a promising method to further enhance the phosphorescence property of PVA-IPA, leading to the co-doping material PVA-IPA-ClCH2COOH with ultralong phosphorescence lifetime as 500 ms and high phosphorescence quantum yield (ΦP) as 23.7%. Depending on these promising phosphorescence behaviors, PVA-IPA-ClCH2COOH was successfully applied in the fields of information anti‐counterfeiting and artificial light harvest.

Introduction: Limited data exist on the gross motor abilities of children with cystic fibrosis (CF). The objective of this quality improvement project was to implement a systematic gross motor assessment in children with CF ages 4-12 years. Methods: Physical therapists aimed to evaluate at least 50% of eligible children at our CF Center over 1 year using the Bruininks-Oseretsky Test of motor Proficiency, second edition (BOT-2), a norm referenced assessment for gross motor skills, with delays defined by scores less than 18 th percentile. Demographic and clinical data including body mass index, hospitalizations, genotype, and comorbidities were collected. Basic descriptive statistics summarized patient information. Parametric and non-parametric methods compared groups of interest. Linear regression assessed associations between BOT-2 measures and clinical characteristics. Results: Of the 105 eligible children, 72 (69%) completed the BOT-2 over 1 year. Forty-five (62.5%) scored below average in at least one category. Impaired strength (22.2%) was most common, followed by impaired balance (16.7%), running speed and agility (15.3%), and bilateral coordination (8.3%). Eleven (15.5%) scored below average on their total motor composite score (TMC). Increased age, comorbidities and hospitalizations were associated with a lower TMC. Conclusions: The BOT-2 was successfully implemented as part of routine CF care to screen for gross motor delays. Results suggest that a high percentage of children with CF, especially older children with comorbid conditions or a history of hospitalization, have impaired gross motor function. These findings support the need for routine gross motor evaluations and physical therapy interventions within pediatric CF clinics.

Paediatric home invasive mechanical ventilation patients are a small but resource intensive cohort, requiring close monitoring and multidisciplinary care. Patients are often dependent on their ventilator for life support, with any significant complications such as equipment failure, tracheostomy blockage, or accidental decannulation becoming potentially life threatening, if not identified quickly. This review discusses the indications and variations in practice worldwide, in terms of models of care, including home care provision, choice of equipment and monitoring. With advances in technology, optimal monitoring strategies for home, continue to be debated: In-built ventilator alarms are often inadequately sensitive for paediatric patients, necessitating additional external monitoring devices to minimise risk. Pulse oximetry has been the preferred monitoring modality at home, though in some special circumstances such as congenital central hypoventilation syndrome, home carbon dioxide monitoring may be important to consider. Children should be under regular follow up at specialist respiratory centres where clinical evaluation, nocturnal oximetry and capnography monitoring and/or poly(somno)graphy and analysis of ventilator download data can be performed regularly to monitor progress. Recent exciting advances in technology, particularly in telemonitoring, which have potential to hugely benefit this complex group of patients are also discussed.

Substance use disorders (SUDs) are highly prevalent and have deleterious effects on one’s health and well-being. Inpatient treatment for SUDs reduces patient relapse, which subsequently ameliorates these negative effects on the individual and society. Additionally, those who complete treatment are less likely to relapse compared to those who do not complete treatment. Thus, maintaining patient engagement in treatment and reducing the rates of those leaving against medical advice (AMA) is particularly important. Examining the factors and comorbidities that may contribute to treatment dropout has the potential to identify at-risk patients in need of additional individualized intervention. The current study aimed to examine comorbid anxiety, depression, and post-traumatic stress disorder (PTSD) symptoms as predictors of dropout AMA in a residential substance use treatment population. Results showed that patients with social anxiety were more likely to leave treatment AMA, while those with PTSD were more likely to complete treatment. Findings suggest that PTSD-specific treatment, as offered in this facility, may help with patient retention, while group focused therapy may be distressing to those with social anxiety. Clinical implications of this research may include incorporating evidence-based practice for social anxiety early during inpatient treatment to reduce anxiety such that patients may better engage with SUDs treatment.

Leather and leather products are the most traded commodities and play a significant role in the world’s economy. The traditional tanning methods and the high quality of Indian leathers receive global appreciation. The existing leather manufacturing methods made a significant loss in epidermal constituents which impact the commercial importance of the animal species. Embossing techniques could potentially contribute to an increase in fraudulent activities. Since the fate of genetic material in finished leather is questionable, which poses a challenge to the molecular approach for identification. In order to obviate the problem, the present study aims to recover amplifiable mtDNA from leather products and focus on developing probes and primers for RT-PCR, which significantly enhance cross-specificity. Data mining and phylogenetic analyses of mtDNA genes (12S rRNA, COI, and Cyt b) for Indian Ovis aries skins exhibit the taxonomic positions and the intraspecies relationships, thereby facilitating the probes desigining. The results indicate that the maximum intraspecies genetic divergence observed within 12S rRNA, followed by COI and Cyt b. Since the availability of genetic sequences with reference to Cyt b is comparatively large (602), primers and probes were designed for Cyt b. The RT-PCR analysis of finished Ovis aries leather mtDNA samples yield a ct value in the range of 22–23 cycles, even with a minimum quantity of template DNA as low as 0.3 ng. Results on the cross-specificity analysis are highly encouraging. Hence, it is feasible to identify genuine Ovis aries leather using mtDNA instilling confidence in the export of leather.

Phenotypic covariation among suites of traits may constrain or promote diversification both within and between species, yet few studies have empirically investigated this relationship. In this study we investigate whether phenotypic covariation of craniofacial traits is associated with diversification in an adaptive radiation of pupfishes found only on San Salvador Island, Bahamas. The full radiation of pupfishes includes generalist, durophagous, and lepidophagous pupfish species. We compare phenotypic variation and covariation (i.e., P matrix) between 1) allopatric populations of generalist pupfish from neighboring islands and estuaries in the Caribbean, 2) San Salvador Island pupfish populations not containing the full radiation of fishes, and 3) San Salvador Island pupfish populations containing the full radiation in sympatry. Additionally, we explore whether phenotypic covariation varies between purebred and hybrid pupfish. We found that the P matrix of SSI generalist populations not found in sympatry with specialists exhibited higher levels of mean trait correlation, higher levels of constraints, and lower levels of flexibility compared to generalist populations on other Caribbean islands and sympatric populations of all three species found on SSI. We also documented significant differences between hybrid and purebred phenotypic covariation, where hybrids displayed lower correlations between traits and higher levels of flexibility, which may produce differential fitness in the wild. Ultimately, this study suggests that differences in phenotypic covariation significantly contribute to producing and maintaining organismal diversity.

Soil fungi are involved in the decomposition of organic matter, and they alter soil structure and physicochemical properties and drive the material cycle and energy flow in terrestrial ecosystems. Grazing exclusion is one of the most common measures used to restore degraded grasslands worldwide. However, changes in soil fungal community characteristics during grazing exclusion in different types of grasslands are unknown. Here, we investigated the effects of a 9-year grazing exclusion on soil properties, fungal community composition and diversity in three grassland types (temperate desert, temperate steppe and mountain meadow). The results showed that in the 0-5 cm soil layer, grazing exclusion significantly affected the physicochemical parameters of all three studied grassland types. In the 5-10 cm soil layer, grazing exclusion significantly increased total phosphorus (TP) in temperate deserts by 34.1%, while significantly decreasing bulk density (BD) by 9.8% and the nitrogen: phosphorus ratio (N:P) by 47.1%. The soil fungal community composition differed among the grassland types, For example, significant differences were found among the three grassland types for the Glomeromycota and Mucoromycota, Grazing exclusion, grassland type, and the interaction between the two did not significantly affect soil fungal α-diversity or community composition(P > 0.05), but significantly altered fungal β-diversity (P < 0.05). Overall, our results highlight the importance of soil nutrient content, especially soil Kjeldahl nitrogen, total phosphorus and organic carbon on fungal diversity, and the results provide key insights into how soil fungi respond to grazing exclusion in different grassland types.

Title PageTitle : Comment on: Psychosocial risk and health care utilization in pediatric sickle cell diseaseArticle type : Letter to the editorKeywords: sickle cell disease; psychosocial risk; healthcare utilizationWords count: 441Correspondence: Saifullah Jamil

：The Qinghai−Tibet Plateau (QTP) is among the most sensitive regions to global environmental change worldwide. Although the climate change and engineering construction on the QTP have jointly modified the regional vegetation activity, little is known about how vegetation variation responds. Using Moderate Resolution Imaging Spectroradiometer (MODIS) enhanced vegetation index (EVI) data during 2000−2021, this study investigated the spatiotemporal variation of vegetation activity and the compound effects of climate change and construction along the G318 highway on the QTP (TG318) through the integration of trend, residual, and partial correlation analyses, as well as structural equation modeling. The results showed that the growing season EVI increased significantly at a rate of about 0.0020/year in the western QTP after reconstruction, but fluctuated in the east. Reconstruction generally had a significant effect on the growing season EVI, with contributions of 7.67%, 19.12%, 18.24%, and −4.15% in different sections of TG318, whereas climate change contributed −10.14% to 8.84% of the total variation. The growing season EVI negatively correlated with snow cover and minimum temperature in humid and sub-humid regions, whereas positively related to vapor pressure in semi-arid regions. Moreover, there existed an obvious lag effect of climate change on the growing season EVI, with lag time generally decreasing from west to east and apparent heterogeneity among different months and regions. These findings would help better understand the environmental impacts along the engineering corridors and provide a scientific basis for ecological conservation in the QTP region.

A document by Yunfei Zou. Click on the document to view its contents.

This paper presents a numerical model for the design, development, and feasibility of thermal energy storage systems. The energy storage was composed of a tank that stores phase change material and internal pipes with heat transfer fluid, coupled to a power block to dispatch electrical energy on a small scale for off-grid industrial applications. Subsequently, the evolution of the temperature in charge/discharge cycles, temperature degradation, and storage efficiency was determined with the appropriate magnitudes and behavior through the resolution of a numerical model. In addition, the proposed energy production plant that connects the system to was implemented in an industrial application for Chile. A numerical model was developed using the finite volumes method to simulate the thermocline performance. As a result, the temperature history reflects stable thermal behavior, low degradation, and high efficiency of approximately 92%, with a storage time increasing up to 13 [hrs] and 384.8 [kWh] capacity. Also, implementation was feasible on a small scale due to its compact, modular, and economically competitive characteristics in Concentrated Solar Power. Finally, the proposed design was proven to be an accurate and reliable alternative for small-scale off-grid mining applications.

Diagnosis of hypertrophic cardiomyopathy accompanied with secondary hypertension - case report

Seeds are an important part of plants, ensuring the continuation of plants’ life and providing nutrient reserves for humans and animals. Seed development is controlled by the interplay of several physiological processes. We applied label-free proteomics to round and wrinkled peas using seeds sampled at five growth stages (4 days after anthesis (DAA), 7DAA, 12DAA, 15DAA, and maturity). Phenotypic results indicated that wrinkled peas had lower starch concentration compared to round peas (29.5% vs. 46.6-55.1%). A total of 4,126 high confident proteins were detected, with 22–26% shared across all sampling times within an entry. Early seed growth stages were characterized by more unique proteins compared to maturity. Two-way ANOVA revealed 1,685 proteins significantly different among samples, of which 722 proteins were characterized into 29 functional classes. The four major classes (comprising over 50 proteins) were protein biosynthesis, protein homeostasis, enzymes, and carbohydrate metabolism. Of the two types of comparisons (time-point and entry-wise), time-point comparisons yielded more differentially abundance proteins (596 proteins in total). Different protein classes exhibited different patterns of change during seed development. For example, cell division related proteins were abundant early in seed development, whereas storage proteins were abundant later in seed development (especially after 12DAA). Compared to the round pea entries, the wrinkled entry had significantly lower abundance of starch branching enzymes, a protein involved in the biosynthesis of amylopectin in starch. In conclusion, the results of this study provide valuable information to improve our understanding of seed development and form the basis for further studies.

Purpose: The purpose of this paper is to evaluate the work done by ASHAs in the upliftment of health services in the selected villages and the improvements in the rural health due to ASHA schemes. Design/methodology/approach: Both primary and secondary sources were used, primary data were collected with the help of self-structured questionnaire using the purposive sampling from 90 respondents of ten villages of Garnai i.e. Gangal, lota Garnai, Upper Garnai, Kriya, Dela, Nagallia, Bishora, Padder, Mulgal Bai, Tunday Tlay by using the with five point likert scale, in-depth interview from the villagers. The PLS technique was used for analyzing the data. Findings: The ASHA worker plays a pivotal role in Reproductive and Child Health care (RCH) and family planning, gain a high degree of acceptability in the study area. The village community members paying attention to the advice given by the ASHA worker and taking the immunization vaccine like DPT, BCG, OPV and TT injections for their children and also seeking their help and guidance regarding their health problems. Counselling and Service Delivery is accepted, hence H1a and H1c is accepted. However, Availability and support services are not significantly contributing to service delivery, hence hypothesis H1b and H1d is rejected. The result indicate that service delivery is impacting quality of health services. Hence H2 is accepted. Research limitations/implications: The study is limited only to one sub centre of Garnai, in future more sub centres can be included for future research. The study can be further extended across various regions, states to establish the validity of NHRM schemes. Originality: The ASHA workers playing a crucial role during the immunization drive and by emphasizing on the role immunization on child survival, promoting reproductive health, safe motherhood, ensure the birth of the healthy child, birth spacing and family planning of the child.

With the development of medicine, the change of disease spectrum and the improvement of diagnosis and treatment, some hereditary diseases are increasingly appearing in the diagnosis of pediatric diseases. We retrospectively analyzed the clinical data and genetic results of 111 children who met the inclusion criteria and visited the research center from January 2015 to December 2019, to improve the clinical awareness of gene detection for the early and accurate diagnosis of hereditary metabolic diseases. The average age of participants who underwent gene testing was 2.45 ± 2.80 years (male:female 1.58:1). The clinical manifestations and laboratory indicators of two children with Wilson’s disease (WD) were atypical and were finally confirmed by gene testing. One of the 15 patients with glycogen storage disease (GSD) had a rare type of GSD XV. Among nine children with Alagille syndrome, 75.0% and 11.1% had new mutations in JAG1 and NOTCH2, respectively. Among nine children with a mutation in UGT1A1, c.211G>A and TATA box mutation sites were the most common. c.851_854del was the most common mutation in Citrin deficiency disease. Progressive familial intrahepatic cholestasis (PFIC) 2 accounted for 66.7% of the nine children with PFIC. The most common mutation in the 14 very early-onset inflammatory bowel disease (VEO-IBD) cases was c.301C>T (p.R101W), followed by c.537G>A (p.T179T). In conclusion, early gene detection can provide accurate diagnosis, precise treatment, and improve disease prognosis for children with atypical clinical symptoms and insignificant changes in biochemical indicators for common genetic metabolic diseases in the pediatric digestive system.

Calf diarrhea is a very common disease syndrome in neonatal calves impacting the dairy industry and animal welfare. The study was aimed to isolate E. coli and Salmonella from diarrheic calves and antimicrobial susceptibility. A cross-sectional study was conducted from January 2020 - June 2021 in dairy farms in Hawassa town. A total of 102 fecal samples were collected from diarrheic calves aged up to 4 months from intensive management. Standard bacteriology revealed; 91 (89.2%) and 5 (4.9%) of the fecal samples were with E. coli and Salmonella, respectively. Animal and environmental risk factors showed; age (OR= 10.79, p< 0.05; OR= 9.41, p< 0.05), body condition score (OR=0.037, p< 0.001; OR= 5.86, p< 0.05), bedding (OR=5.73, p< 0.05; OR=0.60, p< 0.05), housing (OR= 6.30, p< 0.05; OR= 0.58, p< 0.05), first colostrum feeding (OR= 4.69, p< 0.05; OR=5.08, p< 0.05), and house hygiene (OR= 0.20, p< 0.05; OR=6.52, p< 0.05) showed significant association with the occurrence of E. coli and Salmonella, respectively. The antimicrobial study revealed that most of the E. coli isolates were highly susceptible to chloramphenicol (100%) followed by ciprofloxacin (95%) and nalidixic acid (87.5%). However, isolated Salmonella showed 100% susceptibility to nalidixic acid, tetracycline, and nitrofurantoin followed by 80% susceptibility to gentamicin. Both isolates showed high resistance to erythromycin and ampicillin. In conclusion, the detection of high E. coli and relatively low Salmonella isolate in this study could be considered as a potential causative agent of diarrhea in calves leading to improved management practices and hygiene suggestions.

This paper proposes a WLAN 802.11ax multi-standard, dual-band Power Amplifier (PA) designed for wireless communication applications. The PA operates across a range of WLAN standards, including 802.11 a/n/p/ac/ax, within the frequency range of 5.125GHz to 7.050GHz. The proposed PA consists of a cascaded two-stage design, the first stage is a driver stage and the second amplification stage is configured as a differential cascode. On-Chip transformers are used as a balun and for impedance matching. The PA is implemented using UMC 65 nm CMOS technology, with core area occupying 0.24 m m 2 . The results demonstrate slight gain expansion at 6GHz, with gain of 20dB, output 1-dB compression point ( P 1 - dB ) of 24.5dB, and Power Added Efficiency (PAE) of 18.9% for continuous wave (CW) signals. For 802.11ax MCS-11 signal, the PA achieves an average power output ( P out ) of more than 17dBm at Error Vector Magnitude (EVM)≤-35dB, and peak Power Added Efficiency (PAE) of 5.6% across the 5GHz and 6GHz bands. The proposed PA exhibits robust performance in process, voltage, and temperature (PVT) analysis, with the worst corner delivering an average power nearly 10dBm. Most of the recent related literature limits the study of PA for WiFi applications to 6GHz, this paper extends the study upto 7.050GHz.

In May 2022, a cluster of non-travel-related cases of human mpox were reported in the UK. The outbreak has since spread world-wide infecting over 85,000 patients and causing over 100 deaths. Recent data clearly suggest that patients infected with Human Immunodeficiency Virus (HIV) with CD4 counts less than 200 cells per mm 3 suffer significantly worse outcomes than immunocompetent patients. The available countermeasures lack robust clinical data and are deployed based on in vitro and animal studies as well as extrapolations from use against other poxviruses. In many cases, despite administration of these available treatments, initiation of antiretroviral therapy, and management of Immune Reconstitution Inflammatory Syndrome (IRIS), patients die. This review summarizes available data, identifies knowledge gaps and proposes recommendations on the management of severe mpox in PLWH.

Tumor-derived exosomes are crucial for early non-invasive and accurate tumor diagnosis in clinical. The development of highly sensitive, simple and intuitive exosome assays has sparked a research upsurge in clinical diagnostics. Here, we develop a bio-responsive the intelligent DNA hydrogel loaded with CRISPR/Cas12a for universal and ultrasensitive detection of the exosomes. The aptamer serves as the target response unit and switch of the intelligent DNA hydrogel network. The target competitively disintegrates the region of the DNA linkers then Cas12a/crRNA encapsulated in the intelligent DNA hydrogel can be released and activated by the collateral sequence, resulting in a high fluorescent intensity for exosome detection at the detection limit of 119 particles/μL. Moreover, a prefabricated colorimetric tube is made by loading a colorimetric filter membrane on the tube lid and intelligent DNA hydrogel on the tube bottom, which enables one-pot portable colorimetric detection. Without the need for laboratory instruments and professionals, this strategy allows for naked eye detection with LOD as low as 104 particles/μL, and shows great applicability in distinguishing between healthy individuals, pretreatment patients, and post treatment patients after obtaining a testable analyte. Collectively, this study constructs an ultra-sensitive detection platform for exosomes, which enables one-step sensing and dual signal output, making it a promising tool for the application of liquid biopsy based on exosomes in clinical diagnosis.

Objective To evaluate the immune effect of the COVID-19 vaccination in patients with coronary artery disease. Methods One hundred and fifty patients were randomly selected among patients with coronary heart disease, including 100 in the vaccination group and 50 in the control group. A retrospective cohort study was conducted to investigate the infection rate of the two groups of people infected with the SARS-CoV-2 during its outbreak and the occurrence of related symptoms after infection. Results The infection rate of the inoculated group and the control group was 91% and 62%, respectively，and the difference was statistically significant（X 2=4.854，P＜0.05）. The incidence of myalgia was 62.6% and 41.9%, respectively，and the difference was statistically significant（X 2=4.052，P＜0.05）. The probability of nasal congestion and rhinorrhea was 46.2% and 22.6%, respectively，and the difference was statistically significant（X 2=5.347，P＜0.05）. Conclusion On patients with coronary heart disease, the COVID-19 vaccine had a relatively poor immunological response.