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Development of disposable electrode for the detection of mosquito-borne viruses
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  • Fahmida Nasrin,
  • Indra Mwmdi Khoris,
  • Ankan Dutta Chowdhury,
  • Sjakurrizal El Muttaqein,
  • Enoch Y. Park
Fahmida Nasrin
Shizuoka University
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Indra Mwmdi Khoris
Shizuoka University
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Ankan Dutta Chowdhury
Amity University - Kolkata Campus
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Sjakurrizal El Muttaqein
Shizuoka University
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Enoch Y. Park
Shizuoka University

Corresponding Author:park.enoch@shizuoka.ac.jp

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Development of disposable, rapid and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen-printed carbon electrode (SPCE) for the detection of two mosquito-borne viruses. The biosensor fabrication has step-wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)-polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP-PAni /PAni-SPCE via the streptavidin-biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody-antigen interaction, this disposable electrode has designed to detect mosquito-borne infectious viruses, Chikungunya virus (CHIKV) and Zika Virus (ZIKV) separately in a wide linear range of 100 fg/mL to 1 ng/mL with a low detection limit of 1.33 fg/mL and 12.31 fg/mL, respectively.
16 Mar 2023Submitted to Biotechnology Journal
17 Mar 2023Assigned to Editor
17 Mar 2023Submission Checks Completed
17 Mar 2023Reviewer(s) Assigned
19 Mar 2023Review(s) Completed, Editorial Evaluation Pending