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Analysis of Cruciferin Content in Whole Seeds of Brassica napus L. by Near-Infrared Spectroscopy
  • Ashley Ammeter,
  • Kenny So,
  • Rob Duncan
Ashley Ammeter
University of Manitoba

Corresponding Author:ammetera@myumanitoba.ca

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Kenny So
University of Manitoba
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Rob Duncan
University of Manitoba
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Globally, there is an increasing demand for sources of plant-based protein. While Brassica napus L. is an important oilseed crop worldwide, there is also interest in improving its ability to serve as a valuable source of plant-based protein. Cruciferin, a seed storage protein that makes up 60% of the protein found in mature seeds of B. napus, is of interest for human consumption as a source of protein and as an ingredient in food products due to its functional properties. Existing methods for quantification of cruciferin protein are often time consuming and destroy the seed. This study explored the potential for the measurement of cruciferin protein content in whole seeds of B. napus by near-infrared spectroscopy (NIRS), to allow for efficient and non-destructive screening of breeding material. An enzyme-linked immunosorbent assay (ELISA)-based reference method was utilized to assess cruciferin content in a diverse population of B. napus. Scanning of whole seed samples produced spectra that were used to develop NIRS calibration equations. Statistical analysis of the calibration results indicated that the NIRS equations developed are poorly suited for prediction of cruciferin content.
31 Oct 2021Submitted to Journal of the American Oil Chemists' Society
01 Nov 2021Submission Checks Completed
01 Nov 2021Assigned to Editor
16 Nov 2021Reviewer(s) Assigned
26 Dec 2021Review(s) Completed, Editorial Evaluation Pending
04 Jan 2022Editorial Decision: Revise Minor
14 Feb 20221st Revision Received
14 Feb 2022Submission Checks Completed
14 Feb 2022Assigned to Editor
02 Mar 2022Review(s) Completed, Editorial Evaluation Pending
05 Mar 2022Editorial Decision: Revise Minor
22 Apr 20222nd Revision Received
22 Apr 2022Submission Checks Completed
22 Apr 2022Assigned to Editor
02 May 2022Review(s) Completed, Editorial Evaluation Pending
06 May 2022Editorial Decision: Accept
Aug 2022Published in Journal of the American Oil Chemists' Society volume 99 issue 8 on pages 655-664. 10.1002/aocs.12616